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Literature summary extracted from

  • MBarek, N.B.; Audoly, G.; Raoult, D.; Gluschankof, P.
    HIV-2 Protease resistance defined in yeast cells (2006), Retrovirology, 3, 58.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.4.23.47 molecular biology an experimental model system based on the expression of HIV-2 protease in yeast cells is established: HIV-2 protease activity kills the yeast cell, this process can be abolished by inhibiting the viral enzyme activity Human immunodeficiency virus 2

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.23.47 HIV-2 protease is expressed in yeast cells Human immunodeficiency virus 2

Protein Variants

EC Number Protein Variants Comment Organism
3.4.23.47 I54M mutant is resistant toward HIV-2 protease inhibitor lopinavir and saquinavir in yeast Human immunodeficiency virus 2
3.4.23.47 K45R mutant is resistant toward HIV-2 protease inhibitor lopinavir and saquinavir in yeast Human immunodeficiency virus 2
3.4.23.47 L90M IC50: 0.0125 (lopinavir), 0.554 (saquinavir), L90M mutation present in primary HIV-2 isolate, modifies the HIV-2 protease susceptibility to saquinavir but not lopinavir Human immunodeficiency virus 2
3.4.23.47 L99F IC50: 0.0111 (lopinavir), 0.1472 (saquinavir) Human immunodeficiency virus 2
3.4.23.47 M76V mutant is resistant toward HIV-2 protease inhibitor lopinavir and saquinavir in yeast Human immunodeficiency virus 2

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.47 lopinavir
-
 Human immunodeficiency virus 2
3.4.23.47 saquinavir
-
 Human immunodeficiency virus 2

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.47 Human immunodeficiency virus 2
-
-
-

Synonyms

EC Number Synonyms Comment Organism
3.4.23.47 HIV-2 protease
-
 Human immunodeficiency virus 2

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.23.47 0.0111
-
 mutant L99F Human immunodeficiency virus 2 lopinavir
3.4.23.47 0.0125
-
 mutant L90M Human immunodeficiency virus 2 lopinavir
3.4.23.47 0.0166
-
-
 Human immunodeficiency virus 2 lopinavir
3.4.23.47 0.1472
-
 mutant L99F Human immunodeficiency virus 2 saquinavir
3.4.23.47 0.1497
-
-
 Human immunodeficiency virus 2 saquinavir
3.4.23.47 0.554
-
 mutant L90M Human immunodeficiency virus 2 saquinavir